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Missense Mutation in the Chlamydomonas Chloroplast Gene that Encodes the Rubisco Large Subunit
Author(s) -
Robert J. Spreitzer,
Tracy Brown,
Zhixiang Chen,
Donghong Zhang,
Souhail R. AlAbed
Publication year - 1988
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.86.4.987
Subject(s) - chlamydomonas , chlamydomonas reinhardtii , rubisco , mutant , biology , protein subunit , chloroplast , gene , genetics , missense mutation , biochemistry , mutation
The 69-12Q mutant of Chlamydomonas reinhardtii lacks ribulose-1,5-bisphosphate carboxylase activity, but retains holoenzyme protein. It results from a mutation in the chloroplast large-subunit gene that causes an isoleucine-for-threonine substitution at amino-acid residue 173. Considering that lysine-175 is involved in catalysis, it appears that mutations cluster at the active site.

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