A Rapid and Simple Procedure for Purification of Indole-3-Acetic Acid Prior to GC-SIM-MS Analysis | Zendy

Kai-Hsien Chen | Zendy; Anita N. Miller | Zendy; Glenn W. Patterson | Zendy; Jerry D. Cohen | Zendy

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A Rapid and Simple Procedure for Purification of Indole-3-Acetic Acid Prior to GC-SIM-MS Analysis

Author(s) -

Kai-Hsien Chen,

Anita N. Miller,

Glenn W. Patterson,

Jerry D. Cohen

Publication year - 1988

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.86.3.822

Subject(s) - chromatography , acetic acid , chemistry , indole 3 acetic acid , resolution (logic) , indole test , solvent , organic chemistry , biochemistry , computer science , artificial intelligence , auxin , gene

A simple and rapid procedure for the purification of indole-3-acetic acid prior to gas chromatography-selected ion monitoring-mass spectrographic analysis was developed using an amino anion exchange minicolumn and a short high resolution C18 column. Since multiple samples can be prepared at one time, the procedure is more rapid and the sample preparation time is reduced to one-third that normally required. In addition, the final recovery was improved by 40 to 50% over that of a solvent partitioning procedure.

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