Open AccessChlorophyll a Fluorescence Yield as a Monitor of Both Active CO2 and HCO3− Transport by the Cyanobacterium Synechococcus UTEX 625
Author(s) -
Anthony G. Miller,
George S. Espie,
David T. Canvin
Publication year - 1988
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.86.3.655
Subject(s) - synechococcus , chemistry , fluorescence , quenching (fluorescence) , total inorganic carbon , chlorophyll fluorescence , chlorophyll , chlorophyll a , cyanobacteria , yield (engineering) , carbon fibers , photochemistry , photosynthesis , analytical chemistry (journal) , biochemistry , environmental chemistry , carbon dioxide , biology , bacteria , materials science , organic chemistry , genetics , physics , quantum mechanics , composite number , metallurgy , composite material
Simultaneous measurements have been made of inorganic carbon accumulation (by mass spectrometry) and chlorophyll a fluorescence yield of the cyanobacterium Synechococcus UTEX 625. The accumulation of inorganic carbon by the cells was accompanied by a substantial quenching of chlorophyll a fluorescence. The quenching occurred even when CO(2) fixation was inhibited by iodoacetamide and whether the accumulation of inorganic carbon resulted from either active CO(2) or HCO(3) (-) transport. Measurement of chlorophyll a fluorescence yield of cyanobacteria may prove to be a rapid and convenient means of screening for mutants of inorganic carbon accumulation.