Open AccessLipid Saturation Induced Microviscosity Increase Has No Effect on the Reducibility of Flash-Oxidized Cytochrome f in Pea Thylakoids
Author(s) -
Zoltán Gombos,
Klára Barabás,
Ferenc Joó,
László Vı́gh
Publication year - 1988
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.86.2.335
Subject(s) - microviscosity , thylakoid , plastoquinone , electron transport chain , photochemistry , chemistry , cytochrome f , membrane fluidity , biophysics , cytochrome , degree of unsaturation , chloroplast , membrane , biochemistry , biology , chromatography , enzyme , gene
Homogeneous catalytic hydrogenation was used to modify the level of fatty acid unsaturation of thylakoid membranes in the pea chloroplast. Fluidity alteration has been monitored simultaneously using the spin-label probe, 16-doxyl stearate. Even in the case of 30% hydrogenation, no change in the reduction rate of flash-oxidized cytochrome f was observed, in contrast to the fact that the same decrease in the double-bond content of the thylakoid membrane resulted in a pronounced inhibition in the full-chain electron transport. We conclude that the rate of lateral diffusion of reduced plastoquinone is unaffected by the lowering of the fluidity of the thylakoid lipid matrix.