Glucosinolate Biosynthesis | Zendy

Thomas M. Glendening | Zendy; Jonathan E. Poulton | Zendy

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Glucosinolate Biosynthesis

Author(s) -

Thomas M. Glendening,

Jonathan E. Poulton

Publication year - 1988

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.86.2.319

Subject(s) - myrosinase , chemistry , sinigrin , sulfation , dithiothreitol , biochemistry , biosynthesis , enzyme , molar concentration , glucosinolate , chromatography , biology , organic chemistry , botany , brassica

After removal of myrosinase activity by concanavalin A-Sepharose 4B chromatography, cell-free extracts of light-grown cress (Lepidium sativum L.) seedlings, catalyzed the sulfation of desulfobenzylglucosinolate (K(m), 0.23 millimolar) to benzylglucosinolate using PAPS (K(m), 1 millimolar) as sulfur donor. Sulfotransferase activity, which was optimal at pH 9.0, was stimulated by MgCl(2), MnCl(2), beta-mercaptoethanol, and dithiothreitol and was inhibited by ZnSO(4) and SH-reagents. The enzyme also sulfated desulfoallyglucosinolate to allylglucosinolate (sinigrin) but was inactive towards all phenylpropanoids and flavonoids tested.

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