Open AccessConversion of Xanthoxin to Abscisic Acid by Cell-Free Preparations from Bean Leaves
Author(s) -
Ram K. Sindhu,
Daniel C. Walton
Publication year - 1987
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.85.4.916
Subject(s) - phaseolus , abscisic acid , pisum , vigna , enzyme assay , sativum , cycloheximide , cucurbita pepo , cucurbita maxima , enzyme , biology , nad+ kinase , chloroplast , botany , biochemistry , protein biosynthesis , gene
Cell-free extracts from the leaves of Phaseolus vulgaris L. convert xanthoxin to abscisic acid. The enzyme activity in dialyzed or acetone-precipitated extracts shows a strong dependence on either NAD or NADP. The enzyme activity appears to be cytosolic with no significant activity observed in chloroplasts. The activity was observed in extracts from roots of Phaseolus vulgaris, and also in extracts prepared from the leaves of Pisum sativum L., Zea mays L., Cucurbita maxima Duchesne, and Vigna radiata L. Neither water stress nor cycloheximide appear to significantly affect the level of enzyme activity in leaves. No intermediates between xanthoxin and abscisic acid were detected.