Simultaneous Quantitation of Indole 3-Acetic Acid and Abscisic Acid in Small Samples of Plant Tissue by Gas Chromatography/Mass Spectrometry/Selected Ion Monitoring | Zendy

John H. Vine | Zendy; Dominique Noiton | Zendy; Julie A. Plummer | Zendy; Cristina Baleriola-Lucas | Zendy; Michael G. Mullins | Zendy

Open Access

Simultaneous Quantitation of Indole 3-Acetic Acid and Abscisic Acid in Small Samples of Plant Tissue by Gas Chromatography/Mass Spectrometry/Selected Ion Monitoring

Author(s) -

John H. Vine,

Dominique Noiton,

Julie A. Plummer,

Cristina Baleriola-Lucas,

Michael G. Mullins

Publication year - 1987

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.85.2.419

Subject(s) - abscisic acid , chemistry , indole 3 acetic acid , acetic acid , mass spectrometry , ion , protonation , selected ion monitoring , chromatography , gas chromatography , orange (colour) , detection limit , gas chromatography–mass spectrometry , auxin , organic chemistry , biochemistry , food science , gene

Indole 3-acetic acid (IAA) was analyzed in apple, orange, and prune tissue by GC-MS by monitoring the protonated molecular ion of its methyl ester at mass to charge ratio (m/z) 190 together with the major fragment ion at m/z 130 and the corresponding ions from the methyl esters of either [(2)H(4)]IAA (m/z 194, 134) or [(2)H(5)]IAA (m/z 195, 135). Abscisic acid (ABA) was analyzed by monitoring the major fragment ions of its methyl ester at m/z 261 and m/z 247 and the corresponding ions from the methyl ester of [(2)H(3)]ABA (m/z 264, 250). Detection limits for IAA and ABA were 1 and 10 picograms, respectively using standards and 1 nanogram per gram dry weight for both phytohormones in plant tissue.

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