Photosynthesis by Intact Isolated Chloroplasts on Solid Support | Zendy

Zoran G. Cerović | Zendy; John K. Cheesbrough | Zendy; David A. Walker | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Photosynthesis by Intact Isolated Chloroplasts on Solid Support

Author(s) -

Zoran G. Cerović,

John K. Cheesbrough,

David A. Walker

Publication year - 1987

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.84.4.1249

Subject(s) - chloroplast , photosynthesis , biophysics , botany , biology , thylakoid , membrane , chemistry , biochemistry , gene

A new approach to measurements of photosynthesis by isolated chloroplasts has been devised. Intact isolated chloroplasts were trapped in the cavities of membrane filters. The thin layers of chloroplasts so obtained were assayed for O(2) evolution and CO(2) assimilation in leaf-chambers. Photosynthetic gas exchange could be demonstrated to take place either in a closed or a flow-through system. The chloroplasts were morphologically intact as shown by light or scanning electron microscopy and displayed stable rates of photosynthesis in the presence of phosphate and alkaline phosphatase. The methods described open the way to in vitro measurement of photosynthesis, by chloroplasts under conditions more closely resembling those in leaves.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research