Open AccessCalmodulin mRNA in Barley (Hordeum vulgare L.)
Author(s) -
Raymond E. Zielinski
Publication year - 1987
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.84.3.937
Subject(s) - calmodulin , hordeum vulgare , messenger rna , biology , complementary dna , meristem , northern blot , hordeum , population , translation (biology) , rna , microbiology and biotechnology , protein biosynthesis , gene expression , biochemistry , gene , botany , poaceae , enzyme , demography , sociology
Calmodulin is encoded by a 650-nucleotide mRNA in higher plants. This messenger was identified in barley and pea by a combination of in vitro translation and blot hybridization experiments using anti-sense RNA produced from an eel calmodulin cDNA probe. In all plant tissues tested, calmodulin mRNA represents between 0.01 and 0.1% of the total translatable mRNA population. Calmodulin mRNA levels are three- to fourfold higher in the meristematic zone of the first leaf of barley. At all other stages of leaf cell differentiation, calmodulin mRNA levels are nearly identical. During light-induced development in barley leaves, the relative proportion of translatable calmodulin mRNA declines about twofold. Cytoplasmic mRNAs that may encode calmodulin-like proteins were also detected. The levels of several of these putative Ca(2+)-binding protein mRNAs are modulated during the course of light-induced barley leaf cell development.