An Enzyme-Linked Immunosorbent Assay (ELISA) for in Vitro Pollen Growth Based on Binding of a Monoclonal Antibody to the Pollen Tube Surface | Zendy

Philip J. Harris | Zendy; Katy A. Freed | Zendy; Marilyn A. Anderson | Zendy; Jodie A. Weinhandl | Zendy; Adrienne E. Clarke | Zendy

Open Access

An Enzyme-Linked Immunosorbent Assay (ELISA) for in Vitro Pollen Growth Based on Binding of a Monoclonal Antibody to the Pollen Tube Surface

Author(s) -

Philip J. Harris,

Katy A. Freed,

Marilyn A. Anderson,

Jodie A. Weinhandl,

Adrienne E. Clarke

Publication year - 1987

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.84.3.851

Subject(s) - monoclonal antibody , pollen , in vitro , pollen tube , enzyme , chemistry , biology , biochemistry , antibody , microbiology and biotechnology , botany , immunology , pollination

An indirect method of quantifying in vitro pollen growth has been developed. This is based on the finding that a monoclonal antibody (PCBC3), which has a primary specificity for alpha-l-arabinofuranosyl residues, binds to the surface of in vitro grown pollen tubes of the ornamental tobacco, Nicotiana alata. This binding was quantified using an enzyme linked immunosorbent assay (ELISA). The method was used to determine the effects of 2-deoxy-d-glucose and nonanoic acid on in vitro pollen growth.

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