Fucosylation of Xyloglucan: Localization of the Transferase in Dictyosomes of Pea Stem Cells | Zendy

Anne Camirand | Zendy; David A. Brummell | Zendy; Gordon Maclachlan | Zendy

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Fucosylation of Xyloglucan: Localization of the Transferase in Dictyosomes of Pea Stem Cells

Author(s) -

Anne Camirand,

David A. Brummell,

Gordon Maclachlan

Publication year - 1987

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.84.3.753

Subject(s) - xyloglucan , fucose , fucosylation , pisum , xylose , biochemistry , vesicle , etiolation , transferase , chemistry , centrifugation , biology , polysaccharide , membrane , galactose , enzyme , fermentation

Microsomal membranes from elongating regions of etiolated Pisum sativum stems were separated by rate-zonal centrifugation on Renografin gradients. The transfer of labeled fucose and xylose from GDP-[(14)C] fucose and UDP-[(14)C]xylose to xyloglucan occurred mainly in dictyosomeenriched fractions. No transferase activity was detected in secretory vesicle fractions. Pulse-chase experiments using pea stem slices incubated with [(3)H]fucose suggest that xyloglucan chains are fucosylated and their structure completed within the dictyosomes, before being transported to the cell wall by secretory vesicles.

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