Vacuolar/Extravacuolar Distribution of Aminopeptidases in Giant Alga Chara australis and Partial Purification of One Such Enzyme | Zendy

Yuji Moriyasu | Zendy; Katsuhiro Sakano | Zendy; Masashi Tazawa | Zendy

Open Access

Vacuolar/Extravacuolar Distribution of Aminopeptidases in Giant Alga Chara australis and Partial Purification of One Such Enzyme

Author(s) -

Yuji Moriyasu,

Katsuhiro Sakano,

Masashi Tazawa

Publication year - 1987

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.84.3.720

Subject(s) - aminopeptidase , leucine , biochemistry , vacuole , biology , alanine , chara , enzyme , hydrolysis , polyacrylamide gel electrophoresis , amino acid , botany , cytoplasm

The presence of two major aminopeptidases (aminopeptidases I and II) in the giant alga Chara australis was shown using polyacrylamide gel electrophoresis. Partially purified aminopeptidase I had a molecular weight of about 120,000, hydrolyzed both leucine-beta-naphthylamide (pH optimum 6.0) and alanine-beta-naphthylamide (pH optimum 7.5), and was located both inside and outside the vacuole. Aminopeptidase I was inhibited by p-chloromercuribenzoic acid, iodoacetic acid, 1,10-phenanthroline, and N-tosyl-l-phenylalanine chloromethyl ketone. Aminopeptidase II hydrolyzed alanine-beta-naphthylamide but not leucine-beta-naphthylamide and was located only outside the vacuole.

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