Identification of Gibberellins in Norway Spruce (Picea abies [L.] Karst.) by Combined Gas Chromatography-Mass Spectrometry | Zendy

Per Christer Odén | Zendy; Ludger Schwenen | Zendy; Jan E. Graebe | Zendy

Open Access

Identification of Gibberellins in Norway Spruce (Picea abies [L.] Karst.) by Combined Gas Chromatography-Mass Spectrometry

Author(s) -

Per Christer Odén,

Ludger Schwenen,

Jan E. Graebe

Publication year - 1987

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.84.2.516

Subject(s) - picea abies , gibberellin , chromatography , mass spectrometry , chemistry , gas chromatography , high performance liquid chromatography , fractionation , gas chromatography–mass spectrometry , bioassay , radioimmunoassay , botany , biology , biochemistry , genetics

Gibberellins A(1) (GA(1)), A(3) and A(9) were identified from extracts of shoots of 6-month old Norway spruce (Picea abies) seedlings by the use of sequential reverse and normal phase high performance liquid chromatography (HPLC), bioassay, radioimmunoassay (RIA) and combined gas chromatography-mass spectrometry (GC-MS). The bioassay and RIA were used after fractionation by HPLC to detect the GA-containing fractions, which were then examined by GC-MS. The GAs identified are considered to be endogenous.

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