Euphorbia escula L. Root and Root Bud Indole-3-Acetic Acid Levels at Three Phenologic Stages | Zendy

Scott J. Nissen | Zendy; Mike E. Foley | Zendy

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Euphorbia escula L. Root and Root Bud Indole-3-Acetic Acid Levels at Three Phenologic Stages

Author(s) -

Scott J. Nissen,

Mike E. Foley

Publication year - 1987

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.84.2.287

Subject(s) - indole 3 acetic acid , dry weight , bud , euphorbia , biology , acetic acid , botany , horticulture , chemistry , auxin , biochemistry , gene

Endogenous indoleacetic acid (IAA) levels of Euphorbia esula L. primary root and root buds were examined at three phenologic stages. High performance liquid chromatography coupled with fluorescence detection and gas chromatography-mass spectrometry, using (13)C(6)[benzene ring]-indole-3-acetic acid as internal standard, were used to measure root bud free and bound IAA levels in vegetative, full flower, and post-flower plants. Highest levels of free IAA (103 nanograms per gram fresh weight) were found in root buds during full flower. Esterified and amide IAA increased significantly in root buds of full flower and post-flower plants, but were not detectable in root buds of vegetative plants. Primary rootfree IAA was highest in vegetative and full flower plants (34.5 nanograms per gram fresh weight) and decreased by 50% in post-flower plants.

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