Male Germ Unit Isolation from Three Tricellular Pollen Species: Brassica oleracea, Zea mays, and Triticum aestivum | Zendy

Elisabeth MatthysRochon | Zendy; Philippe Vergne | Zendy; S. Detchepare | Zendy; Christian Dumas | Zendy

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Male Germ Unit Isolation from Three Tricellular Pollen Species: Brassica oleracea, Zea mays, and Triticum aestivum

Author(s) -

Elisabeth MatthysRochon,

Philippe Vergne,

S. Detchepare,

Christian Dumas

Publication year - 1987

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.83.3.464

Subject(s) - pollen , biology , sperm , brassica , brassica oleracea , zea mays , botany , germ , germ cell , microbiology and biotechnology , agronomy , biochemistry , gene

A technical procedure is described to follow the in vitro release of the ;male germ unit' and the sperm cells from three tricellular pollen species (Brassica, Zea, and Triticum). The condition of the sperm cell was controlled using light microscopy. In addition, for the first time, the sperm cells viability has been checked by the fluorochromatic reaction test. These preliminary results indicate that this procedure appears to be a prerequisite for the successful preparation of purified viable sperm cells.

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