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Subcellular Metabolite Levels in Spinach Leaves
Author(s) -
Richard Gerhardt,
Mark Stitt,
Hans Walter Heldt
Publication year - 1987
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.83.2.399
Subject(s) - spinach , cytosol , metabolite , sucrose , spinacia , biochemistry , chloroplast , fructose , limiting , chenopodiaceae , vacuole , phosphate , chemistry , differential centrifugation , biology , enzyme , cytoplasm , mechanical engineering , engineering , gene
The alterations of subcellular metabolite levels during the day in spinach leaves have been investigated using nonaqueous density gradient centrifugation to separate chloroplasts, cytosol, and vacuole. The results provide direct evidence for the role of sucrose phosphate synthase and cytosolic fructose 1,6-bisphosphatase in regulating sucrose synthesis in leaves and also show that the phosphate translocator is kinetically limiting in vivo.

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