A Rapid Method to Test for Chloroplast DNA Involvement in Atrazine Resistance | Zendy

Sheila McNally | Zendy; P. Bettini | Zendy; Mireille Sévignac | Zendy; H. Darmency | Zendy; Jacques Gasquez | Zendy; Michel Dron | Zendy

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A Rapid Method to Test for Chloroplast DNA Involvement in Atrazine Resistance

Author(s) -

Sheila McNally,

P. Bettini,

Mireille Sévignac,

H. Darmency,

Jacques Gasquez,

Michel Dron

Publication year - 1987

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.83.2.248

Subject(s) - chloroplast dna , restriction enzyme , biology , atrazine , restriction site , point mutation , genetics , gene , dna , chloroplast , dna sequencing , mutation , agronomy , pesticide

A point mutation in the chloroplast psbA gene at codon 264 resulting in an animo acid substitution (ser-gly) manifests itself as atrazine resistance in all recognized weed species studied to date. The single base substitution overlaps a highly conserved Mae1 restriction site which is present in susceptible but not in resistant plants. This restriction enzyme, recently commercialized, has been used to show that it is now possible to discriminate rapidly between the two biotypes without the need for DNA sequencing.

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