5-Enolpyruvylshikimate 3-Phosphate Synthase, the Target Enzyme of the Herbicide Glyphosate, Is Synthesized as a Precursor in a Higher Plant | Zendy

Heike HolländerCzytko | Zendy; Nikolaus Amrhein | Zendy

Open Access

5-Enolpyruvylshikimate 3-Phosphate Synthase, the Target Enzyme of the Herbicide Glyphosate, Is Synthesized as a Precursor in a Higher Plant

Author(s) -

Heike HolländerCzytko,

Nikolaus Amrhein

Publication year - 1987

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.83.2.229

Subject(s) - enzyme , atp synthase , biology , biochemistry , phosphate , in vitro , glyphosate , messenger rna , herbicide resistance , enzyme assay , microbiology and biotechnology , gene

Cell cultures of Corydalis sempervirens adapted to growth in the presence of 5 millimolar glyphosate overproduce the herbicide's target enzyme, 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, 30- to 40-fold. In vitro translation of total RNA and poly(A)-RNA coupled with immunoprecipitation showed that the protein is synthesized as a precursor of relative molecular weight (M(r)) 53900 +/- 900 as compared to M(r) 45500 +/- 1000 of the mature enzyme. Translatable activity of mRNA for EPSP-synthase in glyphosate-adapted cultures is tenfold higher than in nonadapted cultures.

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