An NADH-Dependent Acetoacetyl-CoA Reductase from Euglena gracilis | Zendy

Mary Lou Ernst-Fonberg | Zendy

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An NADH-Dependent Acetoacetyl-CoA Reductase from Euglena gracilis

Author(s) -

Mary Lou Ernst-Fonberg

Publication year - 1986

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.82.4.978

Subject(s) - euglena gracilis , reductase , isoelectric point , ionic strength , biochemistry , coenzyme a , chemistry , cofactor , enzyme , stereochemistry , organic chemistry , gene , chloroplast , aqueous solution

An NADH-dependent acetoacetyl-CoA reductase from Euglena gracilis variety bacillaris was extensively purified and characterized. Two different isoelectric forms of the reductase with identical characteristics otherwise were found. The reductase was noncompetitively inhibited by acyl carrier protein, K(i) 5.6 micromolar at pH 5.4; this inhibition decreased with increasing pH or ionic strength. Coenzyme A was a competitive inhibitor, K(i) 230 micromolar. Kinetic parameters with respect to acetoacetyl-CoA and NADH were sensitive to changes in pH and ionic strength.

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