A Highly Sensitive Method for Quantitative Determination of Abscisic Acid
Author(s) -
Charles H. Michler,
R. Daniel Lineberger,
Grady W. Chism
Publication year - 1986
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.82.2.600
Subject(s) - derivatization , abscisic acid , derivative (finance) , chromatography , bromide , chemistry , gas chromatography , mass spectrometry , electron capture , organic chemistry , biochemistry , ion , financial economics , economics , gene
An abscisic acid derivative was formed by reaction with pentafluorobenzyl bromide which allowed highly sensitive detection by gas-liquid chromatography with electron capture detection. In comparison to the methyl ester derivative, the pentafluorobenzyl derivative of abscisic acid was four times more sensitive to electron capture detection and was stable at room temperature in the presence of ultraviolet light. Derivatization was rapid and the molecular weight of the new compound was confirmed by gas-liquid chromatography-mass spectrometry.
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