Immunochemical Comparison of Glutamine Synthetases from Some Solanaceae Plants | Zendy

José Ramón Botella | Zendy; Francisco M. Cánovas | Zendy; Concepción Ávila | Zendy; Ignacio Núñez de Castro | Zendy; Victoriano Valpuesta | Zendy

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Immunochemical Comparison of Glutamine Synthetases from Some Solanaceae Plants

Author(s) -

José Ramón Botella,

Francisco M. Cánovas,

Concepción Ávila,

Ignacio Núñez de Castro,

Victoriano Valpuesta

Publication year - 1986

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.82.2.585

Subject(s) - lycopersicon , solanaceae , glutamine synthetase , datura stramonium , biology , ouchterlony double immunodiffusion , isozyme , pectinesterase , polyacrylamide gel electrophoresis , antigenicity , datura , nicotiana , nicotiana tabacum , enzyme , biochemistry , botany , glutamine , pectinase , antigen , amino acid , antiserum , genetics , gene

The presence of different glutamine synthetase isoenzymes in different Solanaceae plants and their relative antigenicities against antiglutamine synthetase from tomato leaf serum were studied. All the plants tested showed one glutamine synthetase isoenzyme except for Mandragora autumnalis, which showed two, after discontinuous polyacrylamide gel electrophoresis and specific in situ assay. Antigenicities were compared by the double immunodiffusion technique. The Nicotiana glauca enzyme showed equal reactivity to that of Lycopersicon esculentum, but its antigenicity was higher than Withania frutescens, Datura stramonium, and Hyoscyamus niger. The study of relative antigenicities permitted differentiation of the glutamine synthetase enzymes from uncultivated species of Solanaceae.

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