Identification of Granule-Bound Starch Synthase in Potato Tubers | Zendy

Greetje H. VosScheperkeuter | Zendy; Wietse de Boer | Zendy; Richard G. F. Visser | Zendy; W. J. Feenstra | Zendy; Bernard Witholt | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Identification of Granule-Bound Starch Synthase in Potato Tubers

Author(s) -

Greetje H. VosScheperkeuter,

Wietse de Boer,

Richard G. F. Visser,

W. J. Feenstra,

Bernard Witholt

Publication year - 1986

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.82.2.411

Subject(s) - amaranth , starch synthase , amaranthus hypochondriacus , amaranthaceae , starch , sodium dodecyl sulfate , biochemistry , solanum tuberosum , chemistry , kilodalton , potato starch , granule (geology) , gel electrophoresis , biology , botany , amylose , gene , amylopectin , paleontology

Starch granules isolated from potato (Solanum tuberosum L.) tubers were extracted with sodium dodecyl sulfate and the extract was analyzed. A major protein with a molecular weight of 60,000 daltons was detected. This protein was purified by preparative sodium dodecyl sulfate-gel electrophoresis and specific antibodies were prepared. The anti-60-kilodalton antibodies obtained (a) cross-reacted with the waxy proteins of both maize (Zea mays L.) and grain amaranth (Amaranthus hypochondriacus L.), and (b) inhibited starch synthase activity in partially digested starch granules of the grain amaranth. This evidence strongly suggests that the major 60-kilodalton protein present in potato starch granules represents the granule-bound starch synthase.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research