A Calcium-Activated Phytase from Pollen of Lilium longiflorum | Zendy

Jonathan J. Scott | Zendy; Frank A. Loewus | Zendy

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A Calcium-Activated Phytase from Pollen of Lilium longiflorum

Author(s) -

Jonathan J. Scott,

Frank A. Loewus

Publication year - 1986

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.82.1.333

Subject(s) - lilium , phytase , phytic acid , divalent , chemistry , chromatography , calcium , biochemistry , botany , food science , enzyme , biology , organic chemistry

A phytase was isolated and partially purified from the pollen of Lilium longiflorum Thumb. Optimum activity was at pH 8.0. The phytase was activated by Ca(2+) and Sr(2+) but not by the other divalent cations tested. Activity was inhibited by ethylenediaminetetraacetate. The phytase had a temperature optimum of 55 to 60 degrees C and an activation energy of about 12,700 calories/mole. Extraction of L. longiflorum pollen with 0.1% Triton X-100 increased recovery of the phytase by nearly 4-fold. The phytase had a molecular weight of about 88,000 as determined by gel filtration chromatography and a K(m) value of 7.2 micromolar for phytic acid in the presence of Ca(2+).

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