The Induction of Ethylene Production from Pear Cell Culture by Cell Wall Fragments | Zendy

Cindy B. S. Tong | Zendy; John M. Labavitch | Zendy; Shang Fa Yang | Zendy

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The Induction of Ethylene Production from Pear Cell Culture by Cell Wall Fragments

Author(s) -

Cindy B. S. Tong,

John M. Labavitch,

Shang Fa Yang

Publication year - 1986

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.81.3.929

Subject(s) - pear , pectinase , ethylene , suspension culture , enzyme , cell culture , digestion (alchemy) , cell wall , chemistry , biochemistry , cell , tissue culture , biology , food science , chromatography , botany , in vitro , catalysis , genetics

Macerase, a pectinase-containing enzyme mixture, was used to digest cell walls isolated from cultured pear cells. Following digestion, the reaction mixture was boiled to inactivate enzymes. Addition of soluble aliquots of the mixture to suspension cultures of pear cells led to a rapid and transient production of ethylene by the cells.

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