Open AccessEnergy Facilitated Na+ Uptake in Excised Corn Roots via 31P and 23Na NMR
Author(s) -
Walter V. Gerasimowicz,
ShuI Tu,
Philip E. Pfeffer
Publication year - 1986
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.81.3.925
Subject(s) - chemistry , cyanide , sodium , reagent , isotopes of sodium , sodium cyanide , ion , membrane , hydrazone , nuclear chemistry , proton nmr , electrochemistry , radiochemistry , inorganic chemistry , biochemistry , medicinal chemistry , stereochemistry , electrode , organic chemistry
The uptake of sodium ions by excised corn root tips (Zea mays L. cv FRB-73) was monitored by (23)Na nuclear magnetic resonance (NMR) methods in the presence of a membrane impermeable shift reagent under different metabolic conditions. In addition, for the first time, the energy status as well as the intracellular pH associated with this influx was concurrently evaluated by (31)P NMR. The rate of sodium ion uptake decreased (in order) as the normal metabolic state was changed by the addition of cyanide, anaerobic condition, and carbonyl cyanide trifluoromethoxy phenyl-hydrazone treatment. The results suggest that the proton electrochemical potential of the plasma membrane may facilitate the influx of Na(+).