Cytokinin Activity Induced by Thidiazuron
Author(s) -
John C. Thomas,
Frank R. H. Katterman
Publication year - 1986
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.81.2.681
Subject(s) - cytokinin , thidiazuron , biology , botany , chemistry , microbiology and biotechnology , biochemistry , auxin , gene , in vitro , micropropagation , explant culture
The diphenylurea derivative thidiazuron induces a variety of cytokinin responses. Levels above 5 x 10(-9) molar and 4 x 10(-7) molar stimulate maximum soybean callus growth and radish cotyledon expansion, respectively. A wider range of dose response related effects follows thidiazuron induced tobacco plant regeneration. Analysis of soybean callus extracts strongly suggests that thidiazuron treatment creates an accumulation and/or synthesis of purine cytokinins, able to induce the growth, expansion and regeneration, mentioned above.
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