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Since environmental pollution by potentially acidic gases such as SO(2) causes proton release inside leaf tissues, homogenates of needles of spruce (Picea abies) and fir (Abies alba) and of leaves of spinach (Spinacia oleracea) and barley (Hordeum vulgare) were titrated and buffer capacities were determined as a function of pH. Titration curves of barley leaves were compared with titration curves of barley mesophyll protoplasts. From the protoplasts, chloroplasts and vacuoles were isolated and subjected to titration experiments. From the titration curves, the intracellular distribution of buffering capacities could be deduced. Buffering was strongly pH-dependent. It was high at the extremes of pH but still significant close to neutrality. Owing to its large size, the vacuole was mainly responsible for cellular buffering. However, on a unit volume basis, the cytoplasm was much more strongly buffered than the vacuole. Potentially acidic gases are trapped in the anionic form. They release protons when trapped. The magnitude of diffusion gradients from the atmosphere into the cells, which determines flux, depends on intracellular pH. In the light, the chloroplast stroma, as the most alkaline leaf compartment, has the highest trapping potential. Acidification of the chloroplast stroma inhibits photosynthesis. The trapping potential of the chloroplast is followed by that of the cytosol. Compared with the cytoplasm, the vacuole possesses little trapping potential in spite of its large size. It is particularly small in the acidic vacuoles of conifer needles. In the physiological pH range (slightly above neutrality), chloroplast buffering was about 1 microequivalents H(+) per milligram chlorophyll per pH unit or 35 microequivalents H(+) per milliliter per pH unit in barley or spinach chloroplasts. This compares with SO(2)-generated H(+) production of somewhat more than 1 microequivalent H(+) per milligram chlorophyll per hour, which results from observed SO(2) uptake of leaves when stomata were open and the atmospheric SO(2) concentration was 0.4 microliters per liter (GE Taylor Jr, DT Tingey 1983 Plant Physiol 72: 237-244). At lower SO(2) concentrations, similar H(+) generation inside the cells requires correspondingly longer exposure times.

Buffer Capacities of Leaves, Leaf Cells, and Leaf Cell Organelles in Relation to Fluxes of Potentially Acidic Gases