Immunological Comparisons of Nitrate Reductase of Different Plant Species Using Monoclonal Antibodies | Zendy

Isabelle Chérel | Zendy; Annie MarionPoll | Zendy; Christian Meyer | Zendy; Pierre Rouzé | Zendy

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Immunological Comparisons of Nitrate Reductase of Different Plant Species Using Monoclonal Antibodies

Author(s) -

Isabelle Chérel,

Annie MarionPoll,

Christian Meyer,

Pierre Rouzé

Publication year - 1986

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.81.2.376

Subject(s) - nitrate reductase , monoclonal antibody , enzyme , biology , epitope , nitrate , antibody , biochemistry , botany , chemistry , ecology , immunology

Six monoclonal antibodies against different epitopes of maize leaf nitrate reductase were used to compare plant nitrate reductases in enzyme linked immunosorbent assay and enzyme activity inhibition tests. The number of cross-reacting antibodies was shown to vary with species according to phylogenetic classification, ranging from five (sugarcane) to one (dicotyledonous species). Cross-reactions were restricted to higher plant nitrate reductases.

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