Plastid DNA Content in a Cultured Soybean Line Capable of Photoautotrophic Growth
Author(s) -
Gordon C. Can,
Sabine Heinhorst,
Arthur Weissbach
Publication year - 1986
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.80.2.601
Subject(s) - plastid , biology , dna , glycine , chloroplast , botany , cell culture , suspension culture , biochemistry , microbiology and biotechnology , gene , genetics , amino acid
The levels of chloroplast DNA in a cultured photoautotrophic soybean (Glycine max [L.] Merr. v Corsoy) cell line were determined by molecular hybridization. The cells were also grown photomixotrophically and heterotrophically as suspension cultures and the level of plastid DNA was found to be constant at approximately 26% of the total cellular DNA in all three growth modes. By comparison, total cellular DNA extracted from plants of the same variety used as the explant source for the cultured cells contained 12.3 to 18.9% (leaves and seeds) and 6.1 to 8.9% (roots) plastid DNA.
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