13C6-[Benzene Ring]-Indole-3-Acetic Acid | Zendy

Jerry D. Cohen | Zendy; Bruce G. Baldi | Zendy; Janet P. Slovin | Zendy

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¹³C₆-[Benzene Ring]-Indole-3-Acetic Acid

Author(s) -

Jerry D. Cohen,

Bruce G. Baldi,

Janet P. Slovin

Publication year - 1986

Publication title -

plant physiology

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.80.1.14

Subject(s) - deuterium , benzene , indole 3 acetic acid , chemistry , indole test , acetic acid , ring (chemistry) , isotope , tritium , chromatography , lemna gibba , isotopes of carbon , stereochemistry , organic chemistry , biochemistry , auxin , biology , quantum mechanics , nuclear physics , gene , ecology , aquatic plant , macrophyte , physics , total organic carbon

Indole-3-acetic acid (IAA) labeled with (13)C in the six carbons of the benzene ring is described for use as an internal standard for quantitative mass spectral analysis of IAA by gas chromatography/selected ion monitoring. [(13)C(6)]IAA was compared to the available deuterium labeled compounds and shown to offer the advantages of nonexchangeability of the isotope label, high isotopic enrichment, and chromatographic properties identical to that of the unlabeled compound. The utility of [(13)C(6)]IAA for measurement of endogenous IAA levels was demonstrated by analysis of IAA in Lemna gibba G-3.

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