A NEW METHOD AND INSTRUMENT FOR THE QUANTITATIVE DETERMINATION OF CHLOROPHYLL | Zendy

R. E. Oltman | Zendy

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A NEW METHOD AND INSTRUMENT FOR THE QUANTITATIVE DETERMINATION OF CHLOROPHYLL

Author(s) -

R. E. Oltman

Publication year - 1933

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.8.2.321

Subject(s) - chlorophyll , remote sensing , environmental science , chemistry , botany , biology , geology

methods, andhasshownthe percentage oferror prevalent ineach. Thecolorimetric methoddependsontheability oftheeyetomatch depths ofcolor, anoperation inwhichthehumaneyeisnotoriously ineffi- cient. Inaddition, this methoddoesnotadequately determine slight differ- ences incolor, thelimit ofsensitivity andaccuracy being twodecimal places. Thespectrophotometric methoddepends onmeasuring the-width ofthe absorption bandsofchlorophyll intheredendofthespectrum. This methodisneither speedy norpractical formakingaconsiderable number ofdeterminations, since foreachdetermination a separate spectrophoto- graphmuLst betaken, whichrequires considerable time.Inaddition, an expensive spectrograph isnecessary, andtheaidofanexpert physicist is advisable forcorrectly interpreting thephotographs. Likethecolorimetric method, itispossible toascertain concentrations ofchlorophyll onlytotwo decimal places. Thepurpose indeveloping theinstrument heredescribed wastoelimi- natethepossibilities ofpersonal error inobservation, todetect moreminute differences thanispossible bythepreceding methods, andtomakethe quantitative determination ofchlorophyll aneasier andsimpler process. Thisinstrument willdetect differences of5mg.between samples, andhas amaximumerror of9percent. atthelowerconcentrations andaminimunm errorof2percent. atthehigher concenitrations, whicherrors inmostcases affect onlly thethird decimal place. Figures 1and2illustrate theapparatus. A photoelectric cell(Weston photronic), whichrequires noexternal resistances, noamplification ofthe 1Presented atthegeneral scientific sessions oftheOhioAcademyi ofScience, Dela-

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