Nitrate Reductase of Green Algae Is Located in the Pyrenoid | Zendy

Antonio LópezRuiz | Zendy; Jean Pierre Verbelen | Zendy; J. M. Roldán | Zendy; Jesús Díez | Zendy

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Nitrate Reductase of Green Algae Is Located in the Pyrenoid

Author(s) -

Antonio LópezRuiz,

Jean Pierre Verbelen,

J. M. Roldán,

Jesús Díez

Publication year - 1985

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.79.4.1006

Subject(s) - nitrate reductase , green algae , chlorella , dunaliella salina , algae , biology , botany , nitrate , chlorella vulgaris , scenedesmus , chlorophyceae , biochemistry , enzyme , dunaliella , chlorophyta , ecology

Antibodies against nitrate reductase from Monoraphidium braunii have been used to determine the antigenic relationships of nitrate reductases from different green algae. Nitrate reductases from Chlamydomonas reinhardii, Chlorella fusca, Dunaliella salina, and Scenedesmus obliquus, were inhibited by, and cross-reacted with, antibodies raised against the enzyme from Monoraphidium braunii.These antibodies were also used to determine, by immunoelectron microscopy, the intracellular location of nitrate reductase in the aforementioned green algae. In all cases, the enzyme was specifically located in the pyrenoid.

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