Separation and Quantitation of Polyamines in Plant Tissue by High Performance Liquid Chromatography of Their Dansyl Derivatives | Zendy

Mary Ann Lila Smith | Zendy; Peter J. Davies | Zendy

Open Access

Separation and Quantitation of Polyamines in Plant Tissue by High Performance Liquid Chromatography of Their Dansyl Derivatives

Author(s) -

Mary Ann Lila Smith,

Peter J. Davies

Publication year - 1985

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.78.1.89

Subject(s) - putrescine , spermidine , cadaverine , spermine , chromatography , polyamine , dansyl chloride , chemistry , high performance liquid chromatography , plant tissue , fluorescence , biochemistry , derivatization , biology , enzyme , botany , physics , quantum mechanics

High performance liquid chromatography in combination with fluorescence spectrophotometry can be used to separate and quantitate polyamines (putrescine, cadaverine, spermidine, spermine), prepared as their dansyl derivatives, from plant tissue. The procedure gives sensitive and consistent results for polyamine determinations in plant tissue. In a standard mixture, the minimal detection level was less than 1 picomole of polyamines.

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