Determination of Endogenous Indole-3-Acetic Acid in Plagiochila arctica (Hepaticae)
Author(s) -
David M. Law,
Dominick V. Basile,
Margaret R. Basile
Publication year - 1985
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.77.4.926
Subject(s) - indole 3 acetic acid , auxin , chemistry , endogeny , mass spectrometry , leafy , acetic acid , chromatography , leafy vegetables , gas chromatography , indole test , greening , botany , biochemistry , biology , food science , ecology , gene
Endogenous indole-3-acetic acid (IAA) was found in axenically cultured gametophytes of the leafy liverwort, Plagiochila arctica Bryhn and Kaal., by high-performance liquid chromatography with electrochemical detection. Identification of the methylated auxin was confirmed by gas chromatography-mass spectrometry. Addition of 57 micromolar IAA to cultures increased relative production of ethylene. This is the first definitive (gas chromatography-mass spectrometry) demonstration of the natural occurrence of IAA in a bryophyte.
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