Accumulation and Subcellular Localization of α-Galactosidase-Hemagglutinin in Developing Soybean Cotyledons | Zendy

Eliot M. Herman | Zendy; Leland M. Shan | Zendy

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Accumulation and Subcellular Localization of α-Galactosidase-Hemagglutinin in Developing Soybean Cotyledons

Author(s) -

Eliot M. Herman,

Leland M. Shan

Publication year - 1985

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.77.4.886

Subject(s) - subcellular localization , hemagglutinin (influenza) , biology , biochemistry , chemistry , botany , gene

We have investigated the accumulation and intracellular localization of soybean (Glycine max [L.] Merr. cv Forrest) alpha-galactosidase-hemagglutinin during seed development. Cotyledon tissue was embedded in Lowicryl K4M and immunocytochemical localization was accomplished through treating thin sections with alpha-galactosidase antisera followed by indirect labeling with protein A coupled to colloidal gold. Gold particles were localized on the Golgi apparatus and protein bodies. We interpret this to indicate that alpha-galactosidase-hemagglutinin is transferred to and transported through the Golgi apparatus and finally deposited within the protein body by a Golgi apparatus-mediated process.

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