Isolation of Mitochondria from Soybean Leaves on Discontinuous Percoll Gradients | Zendy

Terry C. Hrubec | Zendy; J. Michael Robinson | Zendy; Robert P. Donaldson | Zendy

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Isolation of Mitochondria from Soybean Leaves on Discontinuous Percoll Gradients

Author(s) -

Terry C. Hrubec,

J. Michael Robinson,

Robert P. Donaldson

Publication year - 1985

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.77.4.1010

Subject(s) - percoll , alternative oxidase , salicylhydroxamic acid , mitochondrion , peroxisome , cyanide , biology , biochemistry , glycine , differential centrifugation , centrifugation , photorespiration , chemistry , amino acid , enzyme , inorganic chemistry , gene

A technique to isolate mitochondria from chamber-grown soybeans (Glycine max cv Williams) was developed. The mitochondria were isolated by centrifugation on discontinuous Percoll gradients which yielded a sharp band of mitochondria contaminated by only 4% of the total chlorophyll in the gradient. Contamination by peroxisomes was also slight. The isolated mitochondria oxidized malate plus glutamate, NADH, and malate with respiratory control. They also showed cyanide-insensitive, alternative pathway activity which was inhibited by salicylhydroxamic acid.

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