Glutamine Metabolism in Corn Kernels Cultured In Vitro | Zendy

Santosh Misra | Zendy; Ann Oaks | Zendy

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Glutamine Metabolism in Corn Kernels Cultured In Vitro

Author(s) -

Santosh Misra,

Ann Oaks

Publication year - 1985

Publication title -

plant physiology

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.77.3.520

Subject(s) - endosperm , caryopsis , starch , glutamine , glutelin , metabolism , in vitro , chemistry , zea mays , biochemistry , amino acid , botany , biology , food science , storage protein , poaceae , agronomy , gene

The fate of glutamine, the major source of nitrogen supplied to the developing maize endosperm, has been examined in endosperm tissues of corn caryopsis grown under sterile conditions. In the culture system, [U-(14)C]glutamine was included in the medium or was injected directly into the endosperm. Samples were harvested at intervals up to 168 hours. Protein and starch fractions were then separated and analyzed for their (14)C content. At 168 hours, 31% of the total label incorporated in the endosperm was in zein, 15% in glutelin, and 24% in starch. When individual amino acids and sugars in the endosperm powders were analyzed, the (14)C still remaining in the glutamine accounted for only 12 to 14% of the total radioactivity.

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