Conditions for Strict Autotrophic Culture of Tobacco Callus | Zendy

Neil A. McHale | Zendy

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Conditions for Strict Autotrophic Culture of Tobacco Callus

Author(s) -

Neil A. McHale

Publication year - 1985

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.77.1.240

Subject(s) - sucrose , agar , agarose , autotroph , callus , food science , botany , substrate (aquarium) , agar plate , biology , liquid medium , chemistry , biochemistry , chromatography , bacteria , ecology , genetics

Organic gelling agents such as agar and agarose provide a heterotrophic substrate for growth of illuminated tobacco callus. When green cells are incubated in CO(2)-free air on a medium lacking sucrose but solidified with 1% agar, an increase in relative dry weight is sustained through two passages. Similar results with different inoculum sources, and with three brands of agar and two forms of agarose, suggest this is a general phenomenon. A fully autotrophic culture system was developed employing polyurethane pads to support cells in a liquid medium lacking sucrose. Growth was negligible in two passages in CO(2)-free air, and increased with each added increment in CO(2) concentration.

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