Immunochemical Characterization of Nitrate Reductase Forms from Wild-Type (cv Williams) and nr1 Mutant Soybean | Zendy

Paul Robin | Zendy; Luc Streit | Zendy; Wilbur Campbell | Zendy; James E. Harper | Zendy

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Immunochemical Characterization of Nitrate Reductase Forms from Wild-Type (cv Williams) and nr₁ Mutant Soybean

Author(s) -

Paul Robin,

Luc Streit,

Wilbur Campbell,

James E. Harper

Publication year - 1985

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.77.1.232

Subject(s) - nad+ kinase , nitrate reductase , mutant , glycine , wild type , biochemistry , polyacrylamide gel electrophoresis , nitrate , microbiology and biotechnology , chemistry , enzyme , biology , amino acid , gene , organic chemistry

Soybean (Glycine max L. Merr.) leaves contain two forms of nitrate reductase (NR)-NAD(P)H:NR and NADH:NR. Wild-type (cv Williams), nr(1) mutant and an unrelated cultivar (Prize) were grown with either no N source or with nitrate. Crude extracts were assayed for NR activities and the enzyme forms were purified on blue Sepharose. Analyses were done by polyacrylamide gel electrophoresis and ;Western blotting' using antibodies specific for NR. NAD(P)H:NR was identified as the constitutive NR present in wild-type and Prize, but was absent from the mutant. All three soybean lines contained nitrate-inducible NADH:NR with highest activity at pH 7.5. The results showed that NAD(P)H:NR and constitutive NR were one in the same and confirmed the presence of NADH:NR with pH 7.5 optimum.

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