γ-Guanidinobutyraldehyde Dehydrogenase of Vicia faba Leaves | Zendy

Hitoshi Matsuda | Zendy; Yonezo Suzuki | Zendy

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γ-Guanidinobutyraldehyde Dehydrogenase of Vicia faba Leaves

Author(s) -

Hitoshi Matsuda,

Yonezo Suzuki

Publication year - 1984

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.76.3.654

Subject(s) - vicia faba , vicia , dehydrogenase , botany , biology , chemistry , biochemistry , enzyme

gamma-Guanidinobutyraldehyde dehydrogenase was purified 27-fold in 40% yield from extracts of Vicia faba leaves. High specificity exist only for gamma-guanidinobutyraldehyde and gamma-aminobutyraldehyde; the K(m) value was 3.4 micromolar for gamma-guanidinobutyraldehyde, 25 micromolar for gamma-aminobutyraldehyde, and 84 micromolar (case of gamma-guanidinobutyraldehyde) for NAD, respectively. The enzyme had a molecular weight of approximately 83,000. Optimal pH and temperature for activity were 9.5 and 45 degrees C, respectively. The enzyme was inhibited strongly by p-chloromercuribenzoate, N-ethylmaleimide, and zincon (2-carboxy-2'-hydroxy-5'-sulfoformazylbenzene).

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