Flavan-3-ol Biosynthesis | Zendy

Helen A. Stafford | Zendy; Hope H. Lester | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Flavan-3-ol Biosynthesis

Author(s) -

Helen A. Stafford,

Hope H. Lester

Publication year - 1984

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.76.1.184

Subject(s) - dithiothreitol , flavan , catechin , chemistry , biosynthesis , suspension culture , thiol , diol , biochemistry , stereochemistry , organic chemistry , enzyme , cell culture , polyphenol , biology , antioxidant , genetics

Extracts of cell suspension cultures from Douglas fir (Pseudotsuga menziesii) needles catalyzed the production of (+)-catechin (2,3-trans flavan-3-o1) from the 2,3-trans-flavan,3,4-cis-diol (leucocyanidin) in a NADPH-dependent reductase reaction at pH 7.4. Catechin was also produced, along with the 3,4-cis-diol, in a double step reduction from (+)-dihydroquercetin. It was necessary to eliminate any thiol such as 2-mercaptoethanol or dithiothreitol from the extract or assay mixture because these thiols apparently formed thioethers with the 3,4-diols.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research