A Simple Plant Nutrient Solution Purification Method for Effective Removal of Trace Metals Using Controlled Pore Glass-8-Hydroxyquinoline Chelation Column Chromatography | Zendy

D. L. Eskew | Zendy; Ross M. Welch | Zendy; Earle E. Cary | Zendy

Open Access

A Simple Plant Nutrient Solution Purification Method for Effective Removal of Trace Metals Using Controlled Pore Glass-8-Hydroxyquinoline Chelation Column Chromatography

Author(s) -

D. L. Eskew,

Ross M. Welch,

Earle E. Cary

Publication year - 1984

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.76.1.103

Subject(s) - chelation , 8 hydroxyquinoline , chemistry , chromatography , trace (psycholinguistics) , nutrient , column chromatography , inorganic chemistry , organic chemistry , linguistics , philosophy

Column chelation chromatography on controlled pore glass-8-hydroxyquinoline was demonstrated to be a very efficient method for removing trace metal contaminants from concentrated macronutrient salt solutions used to prepare nutrient media. By using (63)Ni and (65)Zn radio-isotopes as tracers, controlled pore glass-8-hydroxyquinoline column packings were found to retain 99.9% of the radiotracer and quantitative recovery of the radioisotopes from these columns was obtained by eluting with 1.2 n HCl. This method has several advantages over liquid-liquid extraction methods of purification which previously have been used in plant micronutrient research.

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