Open AccessPolyproline II Confirmation in the Protein Component of Arabinogalactan-Protein from Lolium multiflorum
Author(s) -
Gerrit-Jan van Holst,
Geoffrey B. Fincher
Publication year - 1984
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.75.4.1163
Subject(s) - endosperm , polyproline helix , lolium multiflorum , circular dichroism , moiety , chemistry , arabinogalactan , component (thermodynamics) , biology , biochemistry , botany , stereochemistry , polysaccharide , physics , peptide , thermodynamics
Circular dichroism spectra of the arabinogalactan-protein from suspension-cultured Lolium multiflorum (ryegrass) endosperm cells demonstrate the presence of polyproline II conformation in the protein moiety of the proteoglycan. Subject to a number of theoretical and practical constraints of the method, it can be estimated that at least 30% of the protein component is in this conformation.