Determination of Compartmented Metabolite Pools by a Combination of Rapid Fractionation of Oat Mesophyll Protoplasts and Enzymic Cycling | Zendy

Rüdiger Hampp | Zendy; Marion Goller | Zendy; H. Füllgraf | Zendy

Open Access

Determination of Compartmented Metabolite Pools by a Combination of Rapid Fractionation of Oat Mesophyll Protoplasts and Enzymic Cycling

Author(s) -

Rüdiger Hampp,

Marion Goller,

H. Füllgraf

Publication year - 1984

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.75.4.1017

Subject(s) - protoplast , avena , fractionation , chloroplast , biochemistry , phosphate , biology , hordeum vulgare , cell fractionation , metabolite , chemistry , chromatography , botany , enzyme , poaceae , gene

In vivo pool sizes of a range of metabolites have been determined in subcellular fractions of darkened and illuminated mesophyll protoplasts of Avena sativa L. These estimations were made by combining a method of rapid protoplast fractionation with enzymic cycling techniques. Results are given for reduced and oxidized pyridine nucleotides, triose phosphates, 3-phosphoglycerate, inorganic phosphate, aspartate, malate, oxaloacetate, glutamate, 2-oxoglutarate, and citrate, from chloroplasts, mitochondria, and a fraction representing the remainder of the protoplast. The results indicate distinct differences of compartmented levels of certain metabolites between darkened and illuminated protoplasts.

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