Enzymic Dissociation of Zea Shoot Cell Wall Polysaccharides | Zendy

Yoji Kato | Zendy; Donald J. Nevins | Zendy

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Enzymic Dissociation of Zea Shoot Cell Wall Polysaccharides

Author(s) -

Yoji Kato,

Donald J. Nevins

Publication year - 1984

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.75.3.740

Subject(s) - arabinoxylan , chemistry , polysaccharide , fraction (chemistry) , starch , shoot , xyloglucan , aqueous solution , cell wall , arabinose , sugar , cellulose , galactan , ferulic acid , chromatography , glycosidic bond , botany , biochemistry , organic chemistry , xylose , enzyme , biology , fermentation

The water-insoluble fraction, obtained after successive treatment of an insoluble fraction of a buffer-homogenate of Zea mays L. hybrid B73 x Mo 17 shoots with 3 molar LiCl and hot water, was treated with alpha-amylase to remove starch. This fraction has been subjected to sugar composition analysis and the major glycosidic linkages were determined. The results suggest that the water-insoluble fraction is mainly composed of arabinoxylan, (1 --> 3), (1 --> 4-)-beta-d-glucan, xyloglucan, (1 --> 4)-galactan, polygalacturonic acid, and cellulose. The insoluble fraction also contained ferulic acid (about 440 micrograms per 50 milligrams dry weight), which was released from the fraction by treatment with aqueous NaOH.Novo Ban 120 from Bacillus subtilis was found to be an appropriate enzyme source for selective dissociation of Zea shoot wall polysaccharides.

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