Suberin Production by Isolated Tomato Fruit Protoplasts | Zendy

Gonella S. R. L. Rao | Zendy; J. H. M. Willison | Zendy; W. M. N. Ratnayake | Zendy

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Suberin Production by Isolated Tomato Fruit Protoplasts

Author(s) -

Gonella S. R. L. Rao,

J. H. M. Willison,

W. M. N. Ratnayake

Publication year - 1984

Publication title -

plant physiology

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.75.3.716

Subject(s) - protoplast , suberin , botany , biology , production (economics) , chemistry , cell wall , economics , macroeconomics

The multilamellar wall secreted by protoplasts isolated from locule tissue of tomato (Lycopersicon esculentum L.) fruit was purified, and an extract was obtained after depolymerization with BF(3)-methanol. Analysis of this extract using thin layer chromatography demonstrated the presence of fatty acid methyl esters, fatty alcohols, dicarboxylic acid dimethyl esters, and omega-hydroxy acid methyl esters. These components were quantified using an Iatroscan thin layer chromatography-flame ionization detection system. The different chain lengths in each group were identified and quantified using gas chromatography. The results clearly indicated the presence of suberin.

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