Resolution and Reconstitution of Mg-Protoporphyrin IX Monomethyl Ester (Oxidative) Cyclase, the Enzyme System Responsible for the Formation of the Chlorophyll Isocyclic Ring | Zendy

Yum-Shing Wong | Zendy; Paul A. Castelfranco | Zendy

Open Access

Resolution and Reconstitution of Mg-Protoporphyrin IX Monomethyl Ester (Oxidative) Cyclase, the Enzyme System Responsible for the Formation of the Chlorophyll Isocyclic Ring

Author(s) -

Yum-Shing Wong,

Paul A. Castelfranco

Publication year - 1984

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.75.3.658

Subject(s) - cucumis , protoporphyrin ix , chlorophyll , chloroplast , enzyme , oxidative phosphorylation , cyclase , chemistry , biochemistry , ring (chemistry) , biology , botany , organic chemistry , gene , photodynamic therapy

Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase, the system responsible for the formation of the chlorophyll isocyclic ring in developing cucumber (Cucumis sativus L. cv Beit Alpha) chloroplasts, was resolved into two enzymic components: a high-speed supernatant and a membrane pellet. This reconstituted enzyme system required reduced pyridine nucleotide for activity.

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