A Rapid, Sensitive Method for Quantitating Subunits in Purified Ribulose Bisphosphate Carboxylase Preparations | Zendy

T. John Andrews | Zendy; Beth Ballment | Zendy

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A Rapid, Sensitive Method for Quantitating Subunits in Purified Ribulose Bisphosphate Carboxylase Preparations

Author(s) -

T. John Andrews,

Beth Ballment

Publication year - 1984

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.75.2.508

Subject(s) - ribulose 1,5 bisphosphate , pyruvate carboxylase , size exclusion chromatography , rubisco , protein subunit , sodium dodecyl sulfate , chemistry , gel electrophoresis , biochemistry , chromatography , ribulose , polyacrylamide gel electrophoresis , enzyme , gene

Studies of the interactions of the large and small subunits of ribulose bisphosphate carboxylase require a knowledge of the concentrations of the subunits present in various preparations and their ratio. Since existing sodium dodecyl sulfate-gel electrophoresis procedures proved quantitatively unreliable, a technique based on high performance-gel filtration was developed. The latter is most reliable, takes only about 30 minutes to perform, and detects a minimum of 0.25 micrograms of each subunit.

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