A Rapid Reverse Phase Evaporation Method for the Reconstitution of Uncharged Thylakoid Membrane Lipids That Resist Hydration | Zendy

Sallie G. Sprague | Zendy; L. Andrew Staehelin | Zendy

Open Access

A Rapid Reverse Phase Evaporation Method for the Reconstitution of Uncharged Thylakoid Membrane Lipids That Resist Hydration

Author(s) -

Sallie G. Sprague,

L. Andrew Staehelin

Publication year - 1984

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.75.2.502

Subject(s) - spinacia , thylakoid , chemistry , spinach , chromatography , membrane , lipid bilayer , bilayer , phase (matter) , vesicle , evaporation , solvent , aqueous solution , analytical chemistry (journal) , chloroplast , biochemistry , organic chemistry , physics , gene , thermodynamics

Comparison of several lipid reconstitution methods showed that they were not equally efficient at transferring the predominant thylakoid lipid, monogalactosyldiglyceride (MG), to the aqueous phase. We report a reverse phase evaporation method that employs Freon 11 as a lipid solvent and is capable of successfully hydrating MG in spinach (Spinacia oleracea L.) at room temperature within minutes. Using this method it is possible to force an equal weight mixture of MG and digalactosyldiglyceride into small bilayer vesicles without the formation of inverted micellar ;lipidic particles' in the membranes.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research