Electrophoretic Transfer as a Technique for the Detection and Identification of Plant Amylolytic Enzymes in Polyacrylamide Gels | Zendy

Genichi Kakefuda | Zendy; Stanley H. Duke | Zendy

Open Access

Electrophoretic Transfer as a Technique for the Detection and Identification of Plant Amylolytic Enzymes in Polyacrylamide Gels

Author(s) -

Genichi Kakefuda,

Stanley H. Duke

Publication year - 1984

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.75.1.278

Subject(s) - dextrin , starch , polyacrylamide gel electrophoresis , amylase , polyacrylamide , chemistry , isozyme , biochemistry , electrophoresis , alpha amylase , enzyme , chromatography , isoamylase , gel electrophoresis , glycogen debranching enzyme , glycogen phosphorylase , polymer chemistry

An electrophoretic transfer technique was developed for the specific identification of isozymes of starch debranching enzyme, alpha-amylase, and beta-amylase. Amylolytic enzymes are separated by native polyacrylamide slab gel electrophoresis and proteins in gels are electrophoretically transferred through starch-containing polyacrylamide gels. Each amylolytic enzyme degrades starch in the transfer gel to its characteristic limit dextrin as it moves through the gel. Various limit dextrins in the starch gel are identified by their characteristic color development in KI/I solution. Isozymes of starch debranching enzyme, alpha-amylase, and beta-amylase can be easily identified in the same gel.

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